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However, FMT's mechanism of action in psychiatric disorders remains uncertain, donor screening and long-term safety require further clarification, and at present, no recommendations can be made for its routine use outside of clinical research

Materials and methods Cell culture and transient transfection HeLa, 293FT, DU145, and H1299 cells were cultured in Dulbeccos modified Eagles medium (DMEM, WelGENE) supplemented with 10% fetal bovine serum (FBS, HyClone) and 1% penicillin-streptomycin (Gibco)

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The data reported in this paper will be shared by the lead contact upon reasonable request

After ACK lysis to remove RBCs, the CD8 + T cells, B cells and CD11c + cells were bead-depleted to leave primarily CD4 + T cells